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RPS27L Polyclonal antibody

RPS27L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15871-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein eS27-like, RPS, Ribosomal protein eS27-like, 40S ribosomal protein S27-like

验证数据展示

  • IP experiment of SH-SY5Y using 15871-1-AP

    IP experiment of SH-SY5Y using 15871-1-AP

    IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.

  • WB analysis of SH-SY5Y using 15871-1-AP

    WB analysis of SH-SY5Y using 15871-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 15871-1-AP

    IF Staining of SH-SY5Y using 15871-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 15871-1-AP

    IHC staining of human stomach using 15871-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, human heart tissue, HepG2 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15871-1-AP targets RPS27L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPS27L fusion protein Ag8628 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S27-like
Synonyms Small ribosomal subunit protein eS27-like, RPS, Ribosomal protein eS27-like, 40S ribosomal protein S27-like
Calculated Molecular Weight 84 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC003667
Gene Symbol RPS27L
Gene ID (NCBI) 51065
RRIDAB_2253903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ71UM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPS27L antibody 15871-1-APDownload protocol
IHC protocol for RPS27L antibody 15871-1-APDownload protocol
IF protocol for RPS27L antibody 15871-1-APDownload protocol
IP protocol for RPS27L antibody 15871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates

Authors - Adele Francis Xu
View Article
humanWB

J Mol Biol

Defective Human SRP Induces Protein Quality Control and Triggers Stress Response

Authors - Elena B Tikhonova
View Article