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  • Featured Product
  • KD/KO Validated

RPS3A Polyclonal antibody, PBS Only

RPS3A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14123-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 14123-1-AP (same clone as 14123-1-PBS)

    WB analysis using 14123-1-AP (same clone as 14123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 14123-1-AP (same clone as 14123-1-PBS)

    IHC staining of human colon using 14123-1-AP (same clone as 14123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14123-1-AP (RPS3A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS3A antibody included
  • IF Staining of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    IF Staining of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (14123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    FC experiment of HepG2 using 14123-1-AP (same clone as 14123-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human RPS3A (14123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14123-1-AP (same clone as 14123-1-PBS)

    IP experiment of HeLa using 14123-1-AP (same clone as 14123-1-PBS)

    IP result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:6000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 14123-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RPS3A Ready-To-Use IHC Kit. RPS3A primary antibody included.

产品信息

14123-1-PBS targets RPS3A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPS3A fusion protein Ag5273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S3A
Synonyms
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC066926
Gene Symbol RPS3A
Gene ID (NCBI) 6189
RRIDAB_2253921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61247
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors