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RPS4X Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.391-25 ng/mL

Cat No : MP01754-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01754-3, Human RPS4X Recombinant Matched Antibody Pair - PBS only. 85040-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6513. 85040-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

    Sandwich ELISA standard curve of MP01754-3

    Sandwich ELISA standard curve of MP01754-3, Human RPS4X Recombinant Matched Antibody Pair - PBS only. 85040-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6513. 85040-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • The mean RPS4X concentration was determined to be 91.01 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load, 66.99 ng/mL in U2OS cell extract based on a 1.40 mg/mL extract load and 72.80 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load.

    Sample test of MP01754-3

    The mean RPS4X concentration was determined to be 91.01 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load, 66.99 ng/mL in U2OS cell extract based on a 1.40 mg/mL extract load and 72.80 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01754-3 targets RPS4X in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.391-25 ng/mL (Sandwich ELISA);

Capture antibody: SCAR Recombinant antibody, PBS Only (Capture) 85040-4-PBS (242697H7). 100 μg. Concentration 1 mg/ml.

Detection antibody: SCAR Recombinant antibody, PBS Only (Detector) 85040-2-PBS (242697F1). 100 μg. Concentration 1 mg/ml.

Alternative RPS4X matched antibody pairs: MP01754-1, MP01754-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 85040-4-PBS
Tested Reactivity human
Immunogen SCAR fusion protein Ag6513
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S4, X-linked
Synonyms RPS4X, Small ribosomal subunit protein eS4, X isoform, 40S ribosomal protein S4, 242697H7
GenBank accession numberBC000472
Gene symbol RPS4X
UniprotP62701
Gene ID (NCBI) 6191
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 85040-2-PBS
Tested Reactivity human
Immunogen SCAR fusion protein Ag6513
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S4, X-linked
Synonyms RPS4X, Small ribosomal subunit protein eS4, X isoform, 40S ribosomal protein S4, 242697F1
GenBank accession numberBC000472
Gene symbol RPS4X
UniprotP62701
Gene ID (NCBI) 6191
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C