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RRAS2 Polyclonal antibody

RRAS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12530-1-AP

Print datasheet

Synonyms

TC21, Ras-related protein R-Ras2, Ras-like protein TC21, Ras related protein R Ras2, Ras like protein TC21

验证数据展示

  • WB analysis of NIH/3T3 using 12530-1-AP

    WB analysis of NIH/3T3 using 12530-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 12530-1-AP

    IHC staining of mouse heart using 12530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRAS2 antibody included
  • IHC staining of mouse heart using 12530-1-AP

    IHC staining of mouse heart using 12530-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRAS2 antibody included
  • IP experiment of NIH/3T3 using 12530-1-AP

    IP experiment of NIH/3T3 using 12530-1-AP

    IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug.

  • FC experiment of A431 using 12530-1-AP

    FC experiment of A431 using 12530-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 12530-1-AP

    IF Staining of A431 using 12530-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RRAS2 antibody (12530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse pancreas tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse heart tissue, human heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RRAS2 Ready-To-Use IHC Kit. RRAS2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12530-1-AP targets RRAS2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, monkey
Immunogen RRAS2 fusion protein Ag3226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name related RAS viral (r-ras) oncogene homolog 2
Synonyms TC21, Ras-related protein R-Ras2, Ras-like protein TC21, Ras related protein R Ras2, Ras like protein TC21
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC013106
Gene Symbol RRAS2
Gene ID (NCBI) 22800
RRIDAB_2180206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62070
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRAS2 antibody 12530-1-APDownload protocol
IHC protocol for RRAS2 antibody 12530-1-APDownload protocol
IF protocol for RRAS2 antibody 12530-1-APDownload protocol
IP protocol for RRAS2 antibody 12530-1-APDownload protocol
FC protocol for RRAS2 antibody 12530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.

Authors - Ping Liu
View Article
monkeyWB

Fertil Steril

Long-term effects of repeated superovulation on ovarian structure and function in rhesus monkeys.

Authors - Guoying Dong
View Article
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková
View Article