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  • Featured Product
  • KD/KO Validated

RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22015-1-AP

Print datasheet

Synonyms

180 kDa ribosome receptor homolog, ES/130, ES/130 related protein, ES130, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

验证数据展示

  • WB analysis using 22015-1-AP

    WB analysis using 22015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • FC experiment of BxPC-3 using 22015-1-AP

    FC experiment of BxPC-3 using 22015-1-AP

    1X10^6 BxPC-3 cells were stained with 0.2ug RRBP1 antibody (22015-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

  • IHC staining of human lung cancer using 22015-1-AP

    IHC staining of human lung cancer using 22015-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRBP1 antibody included
  • IHC staining of human lung cancer using 22015-1-AP

    IHC staining of human lung cancer using 22015-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRBP1 antibody included
  • IHC staining of human lung cancer using 22015-1-AP

    IHC staining of human lung cancer using 22015-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRBP1 antibody included
  • IF Staining of HepG2 using 22015-1-AP

    IF Staining of HepG2 using 22015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 22015-1-AP

    IF Staining of NIH/3T3 using 22015-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 22015-1-AP

    IF Staining of NIH/3T3 using 22015-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 22015-1-AP

    IF Staining of HepG2 using 22015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse pancreas tissue, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HepG2 cells
Positive FC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22015-1-AP targets RRBP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RRBP1 fusion protein Ag16648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Synonyms 180 kDa ribosome receptor homolog, ES/130, ES/130 related protein, ES130, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
RRIDAB_2878971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

实验方案

Product Specific Protocols
WB protocol for RRBP1 antibody 22015-1-APDownload protocol
IHC protocol for RRBP1 antibody 22015-1-APDownload protocol
IF protocol for RRBP1 antibody 22015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
View Article
humanWB,IF,IP

Mol Oncol

USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.

Authors - Wenqing Wang
  • KD Validated
View Article

Acta Biochim Biophys Sin (Shanghai)

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

Authors - Dayu Huang
View Article
humanWB,IHC

Cancer Manag Res

RRBP1 is highly expressed in prostate cancer and correlates with prognosis.

Authors - Tieqiu Li
View Article
humanWB

Science

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

Authors - Wonyul Jang
  • KD Validated
View Article
humanWB

Front Oncol

RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype

Authors - Rui Chen
  • KD Validated
View Article