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- KD/KO Validated
RRBP1 Polyclonal antibody
RRBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse pancreas tissue, HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22015-1-AP targets RRBP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IP, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | RRBP1 fusion protein Ag16648 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribosome binding protein 1 homolog 180kDa (dog) |
| Synonyms | Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 180 kDa ribosome receptor homolog |
| Calculated Molecular Weight | 1410 aa, 152 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC128577 |
| Gene Symbol | RRBP1 |
| Gene ID (NCBI) | 6238 |
| RRID | AB_2878971 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2E9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RRBP1 antibody 22015-1-AP | Download protocol |
| IHC protocol for RRBP1 antibody 22015-1-AP | Download protocol |
| IF protocol for RRBP1 antibody 22015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
Mol Oncol USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.
| ||
Acta Biochim Biophys Sin (Shanghai) Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation. | ||
Cancer Manag Res RRBP1 is highly expressed in prostate cancer and correlates with prognosis. | ||
Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function
| ||
Front Oncol RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype
|
