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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-22015

Print datasheet

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 180 kDa ribosome receptor homolog

验证数据展示

  • IF Staining of HepG2 using CL594-22015

    IF Staining of HepG2 using CL594-22015

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated RRBP1 antibody (CL594-22015) at dilution of 1:200.

  • FC experiment of HeLa using CL594-22015

    FC experiment of HeLa using CL594-22015

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RRBP1 (CL594-22015) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22015-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-22015 targets RRBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen RRBP1 fusion protein Ag16648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Synonyms Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 180 kDa ribosome receptor homolog
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight 180 kDa, 109 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
RRIDAB_2919866
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ9P2E9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

实验方案

Product Specific Protocols
IF protocol for CL594 RRBP1 antibody CL594-22015Download protocol
FC protocol for CL594 RRBP1 antibody CL594-22015Download protocol
Standard Protocols
Click here to view our Standard Protocols