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RRBP1 Recombinant antibody

RRBP1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230159G10

Cat No : 82890-3-RR

Print datasheet

Synonyms

DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

验证数据展示

  • WB analysis using 82890-3-RR

    WB analysis using 82890-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82890-3-RR (RRBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82890-3-RR

    IF Staining of HeLa using 82890-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-3-RR, Clone: 230159G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity experiments of 82890-3-RR

    Affinity experiments of 82890-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-3-RR against Human RRBP1 were performed. The affinity constant is 3.26 nM.

经过测试的应用

Positive WB detected inHeLa cells, NCI-H1299 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82890-3-RR is RRBP1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen RRBP1 fusion protein Ag16648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Synonyms DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P2E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

实验方案

Product Specific Protocols
WB protocol for RRBP1 antibody 82890-3-RRDownload protocol
IF protocol for RRBP1 antibody 82890-3-RRDownload protocol
FC protocol for RRBP1 antibody 82890-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols