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RRBP1 Recombinant antibody

RRBP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230159G10

Cat no : 82890-3-RR

Print datasheet

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G10, 180 kDa ribosome receptor homolog

验证数据展示

  • WB analysis using 82890-3-RR

    WB analysis using 82890-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82890-3-RR (RRBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 82890-3-RR

    IHC staining of human stomach cancer using 82890-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82890-3-RR (RRBP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with RRBP1 antibody included
  • IF Staining of HeLa using 82890-3-RR

    IF Staining of HeLa using 82890-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-3-RR, Clone: 230159G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 82890-3-RR

    FC experiment of HeLa using 82890-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of U-2 OS using 82890-3-RR

    FC experiment of U-2 OS using 82890-3-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82890-3-RR

    Affinity and Kinetic Characterization of 82890-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-3-RR against Human RRBP1 were performed. The affinity constant is 3.26 nM.

经过测试的应用

Positive WB detected inHeLa cells, NCI-H1299 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, U-2 OS
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82890-3-RR targets RRBP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RRBP1 fusion protein Ag16648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Synonyms Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G10, 180 kDa ribosome receptor homolog
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P2E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

实验方案

Product Specific Protocols
WB protocol for RRBP1 antibody 82890-3-RRDownload protocol
IHC protocol for RRBP1 antibody 82890-3-RRDownload protocol
IF protocol for RRBP1 antibody 82890-3-RRDownload protocol
FC protocol for RRBP1 antibody 82890-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols