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Conjugate
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验证数据展示

  • WB analysis using 68215-1-Ig

    WB analysis using 68215-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IP experiment of A549 using 68215-1-Ig

    IP experiment of A549 using 68215-1-Ig

    IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug.

RTN3 Monoclonal antibody

RTN3 Monoclonal Antibody for WB, IP, ELISA
Cat No. 68215-1-Ig

产品说明书

CloneNo. 1E3H11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IP, ELISA

别名

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68215-1-Ig targets RTN3 in WB, IP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human
免疫原 RTN3 fusion protein Ag13766 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 reticulon 3
别名 ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1
计算分子量 113 kDa
观测分子量25-28 kDa
GenBank蛋白编号BC011394
基因名称 RTN3
Gene ID (NCBI) 10313
RRIDAB_2935303
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95197
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

实验方案

Product Specific Protocols
WB protocol for RTN3 antibody 68215-1-IgDownload protocol
IP protocol for RTN3 antibody 68215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols