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RTN3 Monoclonal antibody, PBS Only (Detector)

RTN3 Monoclonal Antibody for WB, IP, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IP, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3H11

Cat no : 68215-1-PBS

Print datasheet

Synonyms

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

验证数据展示

  • WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

    WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve ofMP50025-1, RTN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68215-2-PBS. Detection antibody: HRP-conjugated 68215-1-PBS. Standard: Ag13766. Range: 0.625-20 ng/mL.

  • IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

    IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

    IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

产品信息

68215-1-PBS targets RTN3 as part of a matched antibody pair:

MP50025-1: 68215-2-PBS capture and 68215-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IP, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen RTN3 fusion protein Ag13766 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name reticulon 3
Synonyms ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight25-28 kDa
GenBank Accession NumberBC011394
Gene Symbol RTN3
Gene ID (NCBI) 10313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95197
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.