CoraLite® Plus 488-conjugated RTN4/NOGO Polyclonal antibody
RTN4/NOGO Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
For other applications, we recommend the unconjugated version of this antibody, 10740-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-10740 targets RTN4/NOGO in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | RTN4/NOGO fusion protein Ag1164 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | reticulon 4 |
Synonyms | ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507 |
Calculated Molecular Weight | 130 kDa |
Observed Molecular Weight | 190-210 kDa, 45 kDa, 22-25 kDa |
GenBank Accession Number | BC007109 |
Gene Symbol | NOGO |
Gene ID (NCBI) | 57142 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NQC3 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 RTN4/NOGO antibody CL488-10740 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |