验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13498-1-AP targets RUFY1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RUFY1 fusion protein Ag4324 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RUN and FYVE domain containing 1 |
| 别名 | RUN and FYVE domain-containing protein 1, RUFY 1, RABIP4, Rab4-interacting protein, La-binding protein 1 |
| 计算分子量 | 708 aa, 80 kDa |
| 观测分子量 | 69 kDa, 80 kDa |
| GenBank蛋白编号 | BC032571 |
| 基因名称 | RUFY1 |
| Gene ID (NCBI) | 80230 |
| RRID | AB_2183747 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96T51 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RUFY1 antibody 13498-1-AP | Download protocol |
| IF protocol for RUFY1 antibody 13498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. | ||
Mol Biol Cell Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. | ||
Mol Biol Cell COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A. | ||
Cancer Sci Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. |