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Host
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    WB analysis of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa cells using 13498-1-AP (same clone as 13498-1-PBS)

    IP experiment of HeLa cells using 13498-1-AP (same clone as 13498-1-PBS)

    IP result of anti-RUFY1 (13498-1-AP for IP and Detection). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    IF Staining of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

RUFY1 Polyclonal antibody, PBS Only

RUFY1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13498-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13498-1-PBS targets RUFY1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RUFY1 fusion protein Ag4324 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RUN and FYVE domain containing 1
别名
计算分子量 708 aa, 80 kDa
观测分子量 69 kDa, 80 kDa
GenBank蛋白编号BC032571
基因名称 RUFY1
Gene ID (NCBI) 80230
RRIDAB_2183747
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T51
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.