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RUFY3 Recombinant antibody

RUFY3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240519E7

Cat no : 83601-2-RR

Print datasheet

Synonyms

RIPx, Rap2-interacting protein x, Rap2 interacting protein x, Protein RUFY3, KIAA0871

验证数据展示

  • WB analysis using 83601-2-RR

    WB analysis using 83601-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83601-2-RR

    FC experiment of A431 using 83601-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83601-2-RR

    Affinity and Kinetic Characterization of 83601-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, SGC-7901 cells, HeLa cells, BGC-823 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83601-2-RR is RUFY3 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RUFY3 fusion protein Ag35558 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RUN and FYVE domain containing 3
Synonyms RIPx, Rap2-interacting protein x, Rap2 interacting protein x, Protein RUFY3, KIAA0871
Calculated Molecular Weight 53 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC051716
Gene Symbol RUFY3
Gene ID (NCBI) 22902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).

实验方案

Product Specific Protocols
WB protocol for RUFY3 antibody 83601-2-RRDownload protocol
FC protocol for RUFY3 antibody 83601-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols