验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, pig brain tissue, K-562 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RUNX1T1 Ready-To-Use IHC Kit. RUNX1T1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67086-1-Ig targets RUNX1T1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | RUNX1T1 fusion protein Ag7893 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | runt-related transcription factor 1; translocated to, 1 (cyclin D-related) |
| Synonyms | Eight twenty one protein, Cyclin-D-related protein, CDR, CBFA2T1, AML1T1 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC005850 |
| Gene Symbol | RUNX1T1 |
| Gene ID (NCBI) | 862 |
| RRID | AB_2882394 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q06455 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RUNX1T1 antibody 67086-1-Ig | Download protocol |
| IHC protocol for RUNX1T1 antibody 67086-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
