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RUNX1T1 Monoclonal antibody, PBS Only

RUNX1T1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G2F7

Cat no : 67086-1-PBS

Print datasheet

Synonyms

Eight twenty one protein, Cyclin-D-related protein, CDR, CBFA2T1, AML1T1

验证数据展示

  • WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

    WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

    WB result of RUNX1T1 antibody (67086-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

    WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

    IHC staining of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RUNX1T1 antibody included
Planning an IHC experiment? We recommend our IHCeasy RUNX1T1 Ready-To-Use IHC Kit. RUNX1T1 primary antibody included.

产品信息

67086-1-PBS targets RUNX1T1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen RUNX1T1 fusion protein Ag7893 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Synonyms Eight twenty one protein, Cyclin-D-related protein, CDR, CBFA2T1, AML1T1
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC005850
Gene Symbol RUNX1T1
Gene ID (NCBI) 862
RRIDAB_2882394
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06455
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.