RUNX2 Recombinant antibody

RUNX2 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 82636-2-RR
CloneNo. 1G5

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IP, ELISA and More (2)

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, Saos-2 cells, C2C12 cells
Positive IP detected inROS1728 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82636-2-RR targets RUNX2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name runt-related transcription factor 2
Synonyms AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight57-60 kDa
GenBank Accession NumberNM_004348
Gene Symbol RUNX2
Gene ID (NCBI) 860
RRIDAB_3086495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13950
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

实验方案

Product Specific Protocols
WB protocol for RUNX2 antibody 82636-2-RRDownload protocol
IP protocol for RUNX2 antibody 82636-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Intractable Rare Dis Res

Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells

Authors - Yongtao Zhang
humanWB

Bone

CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis

Authors - Lei Li
humanWB

Arch Oral Biol

Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis

Authors - Yu Ren
ratWB

J Orthop Surg Res

Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis

Authors - Lifang Zhang
mouse,ratWB,CoIP

Cell Commun Signal

Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling

Authors - Xue-Xue Zhu
ratWB

Mol Biotechnol

Qiang Jin Mixture Promotes Osteogenic Differentiation of MC3T3-E1 Cells via BMP2/Smads Pathway and its Network Pharmacology Study

Authors - Weiyue Gong
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