验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, Saos-2 cells, C2C12 cells |
| Positive IP detected in | ROS1728 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82636-2-RR targets RUNX2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | runt-related transcription factor 2 |
| 别名 | AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57-60 kDa |
| GenBank蛋白编号 | NM_004348 |
| 基因名称 | RUNX2 |
| Gene ID (NCBI) | 860 |
| RRID | AB_3086495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13950 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| IP protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Intractable Rare Dis Res Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells | ||
Bone CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis | ||
Arch Oral Biol Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis | ||
J Orthop Surg Res Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis | ||
Cell Commun Signal Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling | ||
Mol Biotechnol Qiang Jin Mixture Promotes Osteogenic Differentiation of MC3T3-E1 Cells via BMP2/Smads Pathway and its Network Pharmacology Study |