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验证数据展示

  • WB analysis using 67750-1-Ig

    WB analysis using 67750-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67750-1-Ig

    WB analysis using 67750-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RUVBL1 Monoclonal antibody

RUVBL1 Monoclonal Antibody for WB, ELISA
Cat No. 67750-1-Ig

Print datasheet

CloneNo. 1F2D9

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Synonyms

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HT-1080 cells, HCT 116 cells, K-562 cells, A549 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy RUVBL1 Ready-To-Use IHC Kit. RUVBL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67750-1-Ig targets RUVBL1 in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, rat
Immunogen RUVBL1 fusion protein Ag16688 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RuvB-like 1 (E. coli)
Synonyms 49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A
Calculated Molecular Weight 456 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002993
Gene Symbol RUVBL1
Gene ID (NCBI) 8607
RRIDAB_2918519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y265
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].

实验方案

Product Specific Protocols
WB protocol for RUVBL1 antibody 67750-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols