验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HL-60 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67851 targets RUVBL2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | RUVBL2 fusion protein Ag0253 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | RuvB-like 2 (E. coli) |
别名 | RUVB2, ECP-51, EC:3.6.4.12, CGI-46, 48 kDa TBP-interacting protein |
计算分子量 | 51 kDa |
观测分子量 | 51 kDa |
GenBank蛋白编号 | BC000428 |
基因名称 | RUVBL2 |
Gene ID (NCBI) | 10856 |
RRID | AB_2934575 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9Y230 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 RUVBL2 antibody CL488-67851 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |