CoraLite® Plus 488-conjugated RYR1 Monoclonal antibody
RYR1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B4E12
验证数据展示
经过测试的应用
Positive IF/ICC detected in | PC-12 cells |
For other applications, we recommend the unconjugated version of this antibody, 66539-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66539 targets RYR1 in IF/ICC applications and shows reactivity with Human, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, rat |
Immunogen | RYR1 fusion protein Ag25120 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ryanodine receptor 1 (skeletal) |
Synonyms | CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR |
Calculated Molecular Weight | 565 kDa |
GenBank Accession Number | NM_000540 |
Gene Symbol | RYR1 |
Gene ID (NCBI) | 6261 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P21817 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 RYR1 antibody CL488-66539 | Download protocol |
FC protocol for CL Plus 488 RYR1 antibody CL488-66539 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |