验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-12 cells |
For other applications, we recommend the unconjugated version of this antibody, 66539-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66539 targets RYR1 in IF/ICC applications and shows reactivity with Human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 | RYR1 fusion protein Ag25120 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ryanodine receptor 1 (skeletal) |
| 别名 | CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR |
| 计算分子量 | 565 kDa |
| GenBank蛋白编号 | NM_000540 |
| 基因名称 | RYR1 |
| Gene ID (NCBI) | 6261 |
| RRID | AB_3084256 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P21817 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RYR1 antibody CL488-66539 | Download protocol |
| FC protocol for CL Plus 488 RYR1 antibody CL488-66539 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |