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CoraLite®555-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-30000

Print datasheet

Synonyms

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

验证数据展示

  • FC experiment of MCF-7 using CL555-30000

    FC experiment of MCF-7 using CL555-30000

    1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000), or 0.4 ug CoraLite®555-conjugated EPCAM Rabbit PolyAb (CL555-21050), or unstained (blue). Cells were not fixed.

  • FC experiment of NIH/3T3 using CL555-30000

    FC experiment of NIH/3T3 using CL555-30000

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000) (red), control used were unstained NIH/3T3 cells (Blue). Positive control is CoraLite®555 Calreticulin (CL555-27298) (Green). Cells were fixed with permeabilized with Transcription Factor Staining Buffer Kit (PF00011). and permeabilized Flow Perm Buffer (PF00011).

经过测试的应用

Positive FC (Intra) detected inNIH/3T3 cells
Positive FC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-30000 targets Rabbit IgG control in FC, FC (Intra) applications and shows reactivity with n/a samples.

Tested Applications FC, FC (Intra) Application Description
Tested Reactivity n/a
Immunogen N/A 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rabbit IgG control
Synonyms Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
FC protocol for CL555 Rabbit IgG control antibody CL555-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols