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Rat IGF1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 3.91-250 pg/mL

Cat no : MP01547-3

Synonyms

Igf-1, IGF I, IGFI, IGF-I, Insulin like growth factor I

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01547-3, Rat IGF1 Recombinant Matched Antibody Pair - PBS only. 84782-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1976. 84782-1-PBS was HRP conjugated as the detection antibody. Range: 3.91-250 pg/mL

    Sandwich ELISA standard curve of MP01547-3

    Sandwich ELISA standard curve of MP01547-3, Rat IGF1 Recombinant Matched Antibody Pair - PBS only. 84782-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1976. 84782-1-PBS was HRP conjugated as the detection antibody. Range: 3.91-250 pg/mL

  • Serum of six rats was measured. The IGF1 concentration of detected samples was determined to be 1,325.1 pg/mL with a range of 892.6 - 1,922.3 ng/mL.

    Sample test of MP01547-3

    Serum of six rats was measured. The IGF1 concentration of detected samples was determined to be 1,325.1 pg/mL with a range of 892.6 - 1,922.3 ng/mL.

  • Serum of six mice was measured. The IGF1 concentration of detected samples was determined to be 780.6 pg/mL with a range of 585.0 - 1,089.6 ng/mL.

    Sample test of MP01547-3

    Serum of six mice was measured. The IGF1 concentration of detected samples was determined to be 780.6 pg/mL with a range of 585.0 - 1,089.6 ng/mL.

  • Rat heart tissue (chopped in 1-2 mm piecesin 30 mL of medium) was cultured for 3 days in RPMI supplemented with 10% fetal calfserum and 50 μM β-mercaptoethanol and stimulated with 2.5 ng/mL LPS. J774.A1 cells (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 20 ng/mL IFN-γ, and 1.0 μg/mL LPS for 3 days. Aliquots of the cell culture supernates were removed, assayed for levels of IGF1, and measured 4,740.2 pg/mL in rat heart cell culture supernatant and 28.04 pg/mL in J774A.1 cell culture supernatant.

    Sample test of MP01547-3

    Rat heart tissue (chopped in 1-2 mm piecesin 30 mL of medium) was cultured for 3 days in RPMI supplemented with 10% fetal calfserum and 50 μM β-mercaptoethanol and stimulated with 2.5 ng/mL LPS. J774.A1 cells (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 20 ng/mL IFN-γ, and 1.0 μg/mL LPS for 3 days. Aliquots of the cell culture supernates were removed, assayed for levels of IGF1, and measured 4,740.2 pg/mL in rat heart cell culture supernatant and 28.04 pg/mL in J774A.1 cell culture supernatant.

  • Rat lung tissue and mouse heart tissue were rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for IGF1 and measured 1.6 ng/mL in rat lung tissue homogenate based on a 0.3 mg/mL homogenate load, and 0.7 ng/mL in mouse heart tissue homogenate based on a 1.4 mg/mL homogenate load.

    Sample test of MP01547-3

    Rat lung tissue and mouse heart tissue were rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for IGF1 and measured 1.6 ng/mL in rat lung tissue homogenate based on a 0.3 mg/mL homogenate load, and 0.7 ng/mL in mouse heart tissue homogenate based on a 1.4 mg/mL homogenate load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01547-3 targets IGF1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 3.91-250 pg/mL (Sandwich ELISA);

Capture antibody: Rat IGF1 Recombinant antibody, PBS Only (Capture) 84782-6-PBS (241890G4). 100 μg. Concentration 1 mg/ml.

Detection antibody: Rat IGF1 Recombinant antibody, PBS Only (Detector) 84782-1-PBS (241890A11). 100 μg. Concentration 1 mg/ml.

Alternative IGF1 matched antibody pairs: MP01547-1, MP01547-2

Matched pair protein standard: Eg1976

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84782-6-PBS
Tested Reactivity rat
Immunogen IGF1 fusion protein Eg1976
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor 1
Synonyms
GenBank accession numberAAA41386
Gene symbol Igf1
UniprotP08025
Gene ID (NCBI) 24482
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84782-1-PBS
Tested Reactivity rat
Immunogen IGF1 fusion protein Eg1976
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor 1
Synonyms
GenBank accession numberAAA41386
Gene symbol Igf1
UniprotP08025
Gene ID (NCBI) 24482
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.