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Ribosomal protein L4 Monoclonal antibody, PBS Only

Ribosomal protein L4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C7A8

Cat no : 67028-1-PBS

Print datasheet

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

验证数据展示

  • WB analysis using 67028-1-Ig (same clone as 67028-1-PBS)

    WB analysis using 67028-1-Ig (same clone as 67028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    FC experiment of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

产品信息

67028-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein L4
Synonyms RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009888
Gene Symbol Ribosomal protein L4
Gene ID (NCBI) 6124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.