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S100A14 Recombinant antibody, PBS Only

S100A14 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240528B6

Cat no : 83559-5-PBS

Print datasheet

Synonyms

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

验证数据展示

  • IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A14 antibody included
  • WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

    WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A14 antibody included
  • FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

    FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.

产品信息

The immunogen of 83559-5-PBS is S100A14 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen S100A14 fusion protein Ag0757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A14
Synonyms S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC005019
Gene Symbol S100A14
Gene ID (NCBI) 57402
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7