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S100A14 Recombinant antibody

S100A14 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240528B6

Cat no : 83559-5-RR

Print datasheet

Synonyms

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

验证数据展示

  • IHC staining of human ovary cancer using 83559-5-RR

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A14 antibody included
  • WB analysis using 83559-5-RR

    WB analysis using 83559-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83559-5-RR

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A14 antibody included
  • FC experiment of HeLa using 83559-5-RR

    FC experiment of HeLa using 83559-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

经过测试的应用

Positive WB detected inmouse stomach tissue, rat stomach tissues, human saliva samples
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83559-5-RR is S100A14 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen S100A14 fusion protein Ag0757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A14
Synonyms S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC005019
Gene Symbol S100A14
Gene ID (NCBI) 57402
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

实验方案

Product Specific Protocols
WB protocol for S100A14 antibody 83559-5-RRDownload protocol
IHC protocol for S100A14 antibody 83559-5-RRDownload protocol
FC protocol for S100A14 antibody 83559-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols