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验证数据展示

  • IHC staining of human ovary cancer using 83559-5-RR

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A14 antibody included
  • WB analysis using 83559-5-RR

    WB analysis using 83559-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83559-5-RR

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A14 antibody included
  • FC experiment of HeLa using 83559-5-RR

    FC experiment of HeLa using 83559-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

S100A14 Recombinant antibody

S100A14 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83559-5-RR

产品说明书

CloneNo. 240528B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse stomach tissue, rat stomach tissues, human saliva samples
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83559-5-RR targets S100A14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 S100A14 fusion protein Ag0757 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A14
别名 S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC005019
基因名称 S100A14
Gene ID (NCBI) 57402
RRIDAB_3671175
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HCY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

实验方案

Product Specific Protocols
WB protocol for S100A14 antibody 83559-5-RRDownload protocol
IHC protocol for S100A14 antibody 83559-5-RRDownload protocol
FC protocol for S100A14 antibody 83559-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols