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SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11642-1-AP

Print datasheet

Synonyms

SAF-B2, SAF B2, KIAA0138

验证数据展示

  • WB analysis of MCF-7 using 11642-1-AP

    WB analysis of MCF-7 using 11642-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11642-1-AP

    IHC staining of human breast cancer using 11642-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SAFB2 antibody included
  • IF Staining of U2OS using 11642-1-AP

    IF Staining of U2OS using 11642-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inMCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SAFB2 Ready-To-Use IHC Kit. SAFB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11642-1-AP targets SAFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, rat
Cited Reactivitymouse
Immunogen SAFB2 fusion protein Ag2230 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name scaffold attachment factor B2
Synonyms SAF-B2, SAF B2, KIAA0138
Calculated Molecular Weight528aa,57 kDa; 953aa,107 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC025279
Gene Symbol SAFB2
Gene ID (NCBI) 9667
RRIDAB_2301309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

实验方案

Product Specific Protocols
WB protocol for SAFB2 antibody 11642-1-APDownload protocol
IHC protocol for SAFB2 antibody 11642-1-APDownload protocol
IF protocol for SAFB2 antibody 11642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Proteomics

Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice.

Authors - Bo Zheng
View Article