验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy SAFB2 Ready-To-Use IHC Kit. SAFB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
11642-1-AP targets SAFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | mouse |
| 免疫原 | SAFB2 fusion protein Ag2230 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | scaffold attachment factor B2 |
| 别名 | SAF-B2, SAF B2, KIAA0138 |
| 计算分子量 | 528aa,57 kDa; 953aa,107 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC025279 |
| 基因名称 | SAFB2 |
| Gene ID (NCBI) | 9667 |
| RRID | AB_2301309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| IHC protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| IF protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteomics Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice. | ||
RNA SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells |
