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验证数据展示

  • IF Staining of U2OS using CL488-11642

    IF Staining of U2OS using CL488-11642

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SAFB2 antibody (CL488-11642) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of U2OS using CL488-11642

    FC experiment of U2OS using CL488-11642

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Safb2 Polyclonal Antibody (CL488-11642)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11642

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

SAF-B2, SAF B2, KIAA0138

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SAFB2 Ready-To-Use IHC Kit. SAFB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11642-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11642 targets SAFB2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 SAFB2 fusion protein Ag2230 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 scaffold attachment factor B2
别名 SAF-B2, SAF B2, KIAA0138
计算分子量528aa,57 kDa; 953aa,107 kDa
观测分子量 130 kDa
GenBank蛋白编号BC025279
基因名称 SAFB2
Gene ID (NCBI) 9667
RRIDAB_3672503
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14151
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SAFB2 antibody CL488-11642Download protocol
FC protocol for CL Plus 488 SAFB2 antibody CL488-11642Download protocol
Standard Protocols
Click here to view our Standard Protocols