SAMD14 Polyclonal antibody

SAMD14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 22009-1-AP

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Synonyms

Sterile alpha motif domain-containing protein 14, SAM domain-containing protein 14



经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22009-1-AP targets SAMD14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SAMD14 fusion protein Ag15653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterile alpha motif domain containing 14
Synonyms Sterile alpha motif domain-containing protein 14, SAM domain-containing protein 14
Calculated Molecular Weight 445 aa, 48 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC141822
Gene Symbol SAMD14
Gene ID (NCBI) 201191
RRIDAB_2878969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAMD14 antibody 22009-1-APDownload protocol
IHC protocol for SAMD14 antibody 22009-1-APDownload protocol
IF protocol for SAMD14 antibody 22009-1-APDownload protocol
Standard Protocols
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