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CoraLite® Plus 488-conjugated SAR1B Monoclonal antibody

SAR1B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A1

Cat no : CL488-67765

Print datasheet

Synonyms

GTP binding protein SAR1b, GTP binding protein B, GTBPB, EC:3.6.5.2, CMRD

验证数据展示

  • FC experiment of HEK-293T using CL488-67765

    FC experiment of HEK-293T using CL488-67765

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SAR1B (CL488-67765, Clone:1B11A1) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 67765-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67765 targets SAR1B in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen SAR1B fusion protein Ag7718 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SAR1 homolog B (S. cerevisiae)
Synonyms GTP binding protein SAR1b, GTP binding protein B, GTBPB, EC:3.6.5.2, CMRD
Calculated Molecular Weight 22 kDa
Observed Molecular Weight22-25 kDa
GenBank Accession NumberBC002847
Gene Symbol SAR1B
Gene ID (NCBI) 51128
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6B6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SAR1B antibody CL488-67765Download protocol
Standard Protocols
Click here to view our Standard Protocols