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验证数据展示

  • FC experiment of HEK-293T using CL488-67765

    FC experiment of HEK-293T using CL488-67765

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SAR1B (CL488-67765, Clone:1B11A1) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SAR1B Monoclonal antibody

SAR1B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67765

产品说明书

CloneNo. 1B11A1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

GTP binding protein SAR1b, GTP binding protein B, GTBPB, EC:3.6.5.2, CMRD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 67765-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67765 targets SAR1B in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 SAR1B fusion protein Ag7718 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SAR1 homolog B (S. cerevisiae)
别名 GTP binding protein SAR1b, GTP binding protein B, GTBPB, EC:3.6.5.2, CMRD
计算分子量 22 kDa
观测分子量22-25 kDa
GenBank蛋白编号BC002847
基因名称 SAR1B
Gene ID (NCBI) 51128
RRIDAB_3084377
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6B6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SAR1B antibody CL488-67765Download protocol
Standard Protocols
Click here to view our Standard Protocols