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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28991-1-AP (same clone as 28991-1-PBS)

    WB analysis using 28991-1-AP (same clone as 28991-1-PBS)

    E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28991-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 28991-1-AP (same clone as 28991-1-PBS)

    ELISA experiment of Recombinant protein using 28991-1-AP (same clone as 28991-1-PBS)

    SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm. This data was developed using the same antibody clone with 28991-1-PBS in a different storage buffer formulation.

Virus SARS-CoV-2 S protein (319-541 aa) Polyclonal antibody, PBS Only

SARS-CoV-2 S protein (319-541 aa) Polyclonal Antibody for WB, ELISA, Indirect ELISA
Cat No. 28991-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

virus

应用

WB, ELISA, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28991-1-PBS targets SARS-CoV-2 S protein (319-541 aa) in WB, ELISA, Indirect ELISA applications and shows reactivity with virus samples.

经测试应用 WB, ELISA, Indirect ELISA Application Description
经测试反应性 virus
免疫原 SARS-CoV-2 S protein (319-541 aa) fusion protein Ag30689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SARS-CoV-2 Spike Protein
别名
计算分子量 141 kDa
GenBank蛋白编号NC_045512
基因名称 SARS-COV-2
Gene ID (NCBI) 43740568
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.