CoraLite® Plus 488-conjugated SAT1 Recombinant antibody
SAT1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240157G2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | RAW 264.7 cells |
For other applications, we recommend the unconjugated version of this antibody, 83319-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83319 is SAT1 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | SAT1 fusion protein Ag1154 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | spermidine/spermine N1-acetyltransferase 1 |
| Synonyms | SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1, EC:2.3.1.57 |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 15-25 kDa |
| GenBank Accession Number | BC008424 |
| Gene Symbol | SAT1 |
| Gene ID (NCBI) | 6303 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21673 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SAT1 antibody CL488-83319 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |