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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SBDS Monoclonal antibody

SBDS Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F8A10

Cat no : CL594-67200

Print datasheet

Synonyms

SWDS, Shwachman-Bodian-Diamond syndrome protein, SDS, CGI-97, CGI 97

验证数据展示

  • WB analysis using CL594-67200

    WB analysis using CL594-67200

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67200 (SBDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL594-67200

    FC experiment of HeLa using CL594-67200

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated SBDS Monoclonal antibody (CL594-67200, Clone:1F8A10)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67200-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67200 targets SBDS in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SBDS fusion protein Ag11731 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Shwachman-Bodian-Diamond syndrome
Synonyms SWDS, Shwachman-Bodian-Diamond syndrome protein, SDS, CGI-97, CGI 97
Calculated Molecular Weight 250 aa, 29 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC065700
Gene Symbol SBDS
Gene ID (NCBI) 51119
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3A5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.

实验方案

Product Specific Protocols
WB protocol for CL594 SBDS antibody CL594-67200Download protocol
FC protocol for CL594 SBDS antibody CL594-67200Download protocol
Standard Protocols
Click here to view our Standard Protocols