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验证数据展示

  • WB analysis using CL594-67200

    WB analysis using CL594-67200

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67200 (SBDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL594-67200

    FC experiment of HeLa using CL594-67200

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated SBDS Monoclonal antibody (CL594-67200, Clone:1F8A10)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated SBDS Monoclonal antibody

SBDS Monoclonal Antibody for WB, FC (Intra)
Cat No. CL594-67200

产品说明书

CloneNo. 1F8A10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

SWDS, Shwachman-Bodian-Diamond syndrome protein, SDS, CGI-97, CGI 97

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67200-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67200 targets SBDS in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SBDS fusion protein Ag11731 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Shwachman-Bodian-Diamond syndrome
别名 SWDS, Shwachman-Bodian-Diamond syndrome protein, SDS, CGI-97, CGI 97
计算分子量 250 aa, 29 kDa
观测分子量 29-31 kDa
GenBank蛋白编号BC065700
基因名称 SBDS
Gene ID (NCBI) 51119
RRIDAB_3673516
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3A5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.

实验方案

Product Specific Protocols
WB protocol for CL594 SBDS antibody CL594-67200Download protocol
FC protocol for CL594 SBDS antibody CL594-67200Download protocol
Standard Protocols
Click here to view our Standard Protocols