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SCAMP2 Polyclonal antibody

SCAMP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 15119-1-AP

Print datasheet

Synonyms

Secretory carrier-associated membrane protein 2

验证数据展示

  • IP experiment of HepG2 using 15119-1-AP

    IP experiment of HepG2 using 15119-1-AP

    IP result of anti-SCAMP2 (IP:15119-1-AP, 4ug; Detection:15119-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • WB analysis of HepG2 using 15119-1-AP

    WB analysis of HepG2 using 15119-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 15119-1-AP

    IHC staining of human pancreas cancer using 15119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 15119-1-AP

    IHC staining of human pancreas cancer using 15119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HEK-293 using 15119-1-AP

    IF Staining of HEK-293 using 15119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SCAMP2 antibody (15119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HEK-293 using 15119-1-AP

    FC experiment of HEK-293 using 15119-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug SCAMP2 Polyclonal antibody (15119-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, mouse lung tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15119-1-AP targets SCAMP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SCAMP2 fusion protein Ag7190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretory carrier membrane protein 2
Synonyms Secretory carrier-associated membrane protein 2
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC001376
Gene Symbol SCAMP2
Gene ID (NCBI) 10066
RRIDAB_2184772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.

实验方案

Product Specific Protocols
WB protocol for SCAMP2 antibody 15119-1-APDownload protocol
IHC protocol for SCAMP2 antibody 15119-1-APDownload protocol
IF protocol for SCAMP2 antibody 15119-1-APDownload protocol
IP protocol for SCAMP2 antibody 15119-1-APDownload protocol
FC protocol for SCAMP2 antibody 15119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.

Authors - Feiyu Mao
View Article