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验证数据展示

  • WB analysis using 67932-1-Ig

    WB analysis using 67932-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67932-1-Ig (SCAMP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67932-1-Ig

    IF Staining of HeLa using 67932-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SCAMP3 antibody (67932-1-Ig, Clone: 1C1E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SCAMP3 Monoclonal antibody

SCAMP3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67932-1-Ig

产品说明书

CloneNo. 1C1E6

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, IF/ICC, ELISA

别名

C1orf3, PROPIN1, SCAMP3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, LNCaP cells, A549 cells, Hela cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67932-1-Ig targets SCAMP3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 SCAMP3 fusion protein Ag26073 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 secretory carrier membrane protein 3
别名 C1orf3, PROPIN1, SCAMP3
计算分子量 38 kDa
观测分子量33 kDa
GenBank蛋白编号BC000161
基因名称 SCAMP3
Gene ID (NCBI) 10067
RRIDAB_2918684
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14828
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCAMP3 antibody 67932-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols