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验证数据展示

  • WB analysis using 81468-2-RR (same clone as 81468-2-PBS)

    WB analysis using 81468-2-RR (same clone as 81468-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81468-2-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 81468-2-RR (same clone as 81468-2-PBS)

    FC experiment of HepG2 using 81468-2-RR (same clone as 81468-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 81468-2-RR (same clone as 81468-2-PBS)

    FC experiment of MCF-7 using 81468-2-RR (same clone as 81468-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug SCD Recombinant Antibody (81468-2-RR, Clone:241487B5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01272-1

    Cytometric bead array standard curve of MP01272-1

    Cytometric bead array standard curve of MP01272-1, SCD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81468-2-PBS. Detection antibody: 81468-3-PBS. Standard: Ag29156. Range: 0.313-40 ng/mL.

SCD Recombinant antibody, PBS Only (Capture)

SCD Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 81468-2-PBS

产品说明书

CloneNo. 241487B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase, Acyl CoA desaturase

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SCD Ready-To-Use IHC Kit. SCD primary antibody included.

产品信息

81468-2-PBS targets SCD as part of a matched antibody pair:

MP01272-1: 81468-2-PBS capture and 81468-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SCD fusion protein Ag29156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stearoyl-CoA desaturase (delta-9-desaturase)
别名 Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase, Acyl CoA desaturase
计算分子量 355 aa, 41 kDa
观测分子量28-42 kDa
GenBank蛋白编号BC005807
基因名称 SCD
Gene ID (NCBI) 6319
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00767
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).