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SCN5A Monoclonal antibody, PBS Only

SCN5A Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1C2B3

Cat no : 68273-1-PBS

Print datasheet

Synonyms

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

验证数据展示

  • WB analysis of rabbit heart using 68273-1-Ig (same clone as 68273-1-PBS)

    WB analysis of rabbit heart using 68273-1-Ig (same clone as 68273-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • WB analysis of rat heart using 68273-1-Ig (same clone as 68273-1-PBS)

    WB analysis of rat heart using 68273-1-Ig (same clone as 68273-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 68273-1-Ig (same clone as 68273-1-PBS)

    IHC staining of mouse skeletal muscle using 68273-1-Ig (same clone as 68273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68273-1-Ig (SCN5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68273-1-Ig (same clone as 68273-1-PBS)

    FC experiment of HeLa using 68273-1-Ig (same clone as 68273-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A (68273-1-Ig, Clone:1C2B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

产品信息

68273-1-PBS targets SCN5A in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, rat, mouse, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity rabbit, rat, mouse, human
Immunogen SCN5A fusion protein Ag19275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sodium channel, voltage-gated, type V, alpha subunit
Synonyms Nav1.5, HH1, CMPD2, CMD1E, CDCD2
Calculated Molecular Weight 2016 aa, 227 kDa
Observed Molecular Weight227 kDa
GenBank Accession NumberBC140813
Gene Symbol SCN5A
Gene ID (NCBI) 6331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14524
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).