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验证数据展示

  • WB analysis of rabbit heart using 68273-1-Ig

    WB analysis of rabbit heart using 68273-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 68273-1-Ig

    WB analysis of rat heart using 68273-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 68273-1-Ig

    IHC staining of mouse skeletal muscle using 68273-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68273-1-Ig (SCN5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 68273-1-Ig

    FC experiment of HeLa using 68273-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A (68273-1-Ig, Clone:1C2B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SCN5A Monoclonal antibody

SCN5A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 68273-1-Ig

产品说明书

CloneNo. 1C2B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, FC (Intra), ELISA

别名

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat heart tissue, rabbit heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68273-1-Ig targets SCN5A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 SCN5A fusion protein Ag19275 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sodium channel, voltage-gated, type V, alpha subunit
别名 Nav1.5, HH1, CMPD2, CMD1E, CDCD2
计算分子量 2016 aa, 227 kDa
观测分子量227 kDa
GenBank蛋白编号BC140813
基因名称 SCN5A
Gene ID (NCBI) 6331
RRIDAB_2935356
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14524
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).

实验方案

Product Specific Protocols
WB protocol for SCN5A antibody 68273-1-IgDownload protocol
IHC protocol for SCN5A antibody 68273-1-IgDownload protocol
FC protocol for SCN5A antibody 68273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols