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SCN5A Monoclonal antibody

SCN5A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C2B3

Cat no : 68273-1-Ig

Print datasheet

Synonyms

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

验证数据展示

  • WB analysis of rabbit heart using 68273-1-Ig

    WB analysis of rabbit heart using 68273-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 68273-1-Ig

    WB analysis of rat heart using 68273-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 68273-1-Ig

    IHC staining of mouse skeletal muscle using 68273-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68273-1-Ig (SCN5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 68273-1-Ig

    FC experiment of HeLa using 68273-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A (68273-1-Ig, Clone:1C2B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat heart tissue, rabbit heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68273-1-Ig targets SCN5A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen SCN5A fusion protein Ag19275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sodium channel, voltage-gated, type V, alpha subunit
Synonyms Nav1.5, HH1, CMPD2, CMD1E, CDCD2
Calculated Molecular Weight 2016 aa, 227 kDa
Observed Molecular Weight227 kDa
GenBank Accession NumberBC140813
Gene Symbol SCN5A
Gene ID (NCBI) 6331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).

实验方案

Product Specific Protocols
WB protocol for SCN5A antibody 68273-1-IgDownload protocol
IHC protocol for SCN5A antibody 68273-1-IgDownload protocol
FC protocol for SCN5A antibody 68273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols