CoraLite® Plus 488-conjugated SCN5A Monoclonal antibody
SCN5A Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Rabbit
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C2B3
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68273-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68273 targets SCN5A in FC (Intra) applications and shows reactivity with Human, Rat, Rabbit samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human, Rat, Rabbit |
| Immunogen | SCN5A fusion protein Ag19275 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | sodium channel, voltage-gated, type V, alpha subunit |
| Synonyms | Nav1.5, HH1, CMPD2, CMD1E, CDCD2 |
| Calculated Molecular Weight | 2016 aa, 227 kDa |
| Observed Molecular Weight | 227 kDa |
| GenBank Accession Number | BC140813 |
| Gene Symbol | SCN5A |
| Gene ID (NCBI) | 6331 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14524 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SCN5A antibody CL488-68273 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
