验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68273 targets SCN5A in FC (Intra) applications and shows reactivity with human, rat, rabbit samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, rat, rabbit |
| 免疫原 | SCN5A fusion protein Ag19275 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | sodium channel, voltage-gated, type V, alpha subunit |
| 别名 | Nav1.5, HH1, CMPD2, CMD1E, CDCD2 |
| 计算分子量 | 2016 aa, 227 kDa |
| GenBank蛋白编号 | BC140813 |
| 基因名称 | SCN5A |
| Gene ID (NCBI) | 6331 |
| RRID | AB_3084464 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14524 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SCN5A antibody CL488-68273 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |