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验证数据展示

  • WB analysis using 30037-1-AP

    WB analysis using 30037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30037-1-AP (SCNN1G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SCNN1G Polyclonal antibody

SCNN1G Polyclonal Antibody for WB, ELISA
Cat No. 30037-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, ELISA

别名

Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, PC-3 cells, pig kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30037-1-AP targets SCNN1G in WB, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig
免疫原 SCNN1G fusion protein Ag32176 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sodium channel, nonvoltage-gated 1, gamma
别名 Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg
计算分子量 74 kDa
观测分子量74 kDa
GenBank蛋白编号BC059391
基因名称 SCNN1G
Gene ID (NCBI) 6340
RRIDAB_3669702
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51170
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCNN1G (sodium channel, non-voltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of the kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and may undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).

实验方案

Product Specific Protocols
WB protocol for SCNN1G antibody 30037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols