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SCNN1G Polyclonal antibody

SCNN1G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30037-1-AP

Print datasheet

Synonyms

Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg

验证数据展示

  • WB analysis using 30037-1-AP

    WB analysis using 30037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30037-1-AP (SCNN1G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, PC-3 cells, pig kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30037-1-AP targets SCNN1G in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, pig
Immunogen SCNN1G fusion protein Ag32176 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel, nonvoltage-gated 1, gamma
Synonyms Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg
Calculated Molecular Weight 74 kDa
Observed Molecular Weight74 kDa
GenBank Accession NumberBC059391
Gene Symbol SCNN1G
Gene ID (NCBI) 6340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51170
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCNN1G (sodium channel, non-voltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of the kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and may undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).

实验方案

Product Specific Protocols
WB protocol for SCNN1G antibody 30037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols