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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 31577-1-AP

    WB analysis using 31577-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31577-1-AP (SCRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SCRT1 Polyclonal antibody

SCRT1 Polyclonal Antibody for WB, ELISA
Cat No. 31577-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

SCRT, Scratch 1, hScrt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTT cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31577-1-AP targets SCRT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 SCRT1 fusion protein Ag36215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 scratch homolog 1, zinc finger protein (Drosophila)
别名 SCRT, Scratch 1, hScrt
观测分子量36 kDa
GenBank蛋白编号BC168377
基因名称 SCRT1
Gene ID (NCBI) 83482
RRIDAB_3670042
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BWW7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRT1 (Transcriptional repressor scratch 1), which is expressed in many tissues, especially in brain and adrenal gland. In addition, SCRT1 is also expressed in islet β cells, and its expression is related to insulin secretion ability. SCRT1 is a C2H2 zinc finger transcription inhibitor, which can bind to E-box motif. The encoded protein may promote nerve differentiation and may be related to cancer with neuroendocrine characteristics. The functions of SCRT1 in islet β cells include regulating the expression and secretion of insulin and influencing the proliferation of β cells. SCRT1 may control calcineurin /NFAT signaling pathway by affecting the expression of Syt4, Notch1, Nfatc1 and Nfatc2 genes, which is an important regulator of the growth and function of β cells (PMID: 33309639). The molecular weight of SCRT1 is 36 kDa.

实验方案

Product Specific Protocols
WB protocol for SCRT1 antibody 31577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols