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验证数据展示

  • WB analysis of HepG2 using 16502-1-AP

    WB analysis of HepG2 using 16502-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16502-1-AP

    IP experiment of HepG2 using 16502-1-AP

    IP result of anti-SDCCAG10 (IP:16502-1-AP, 4ug; Detection:16502-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 16502-1-AP

    IF Staining of HepG2 using 16502-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16502-1-AP (SDCCAG10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SDCCAG10 Polyclonal antibody

SDCCAG10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16502-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog, PPIase CWC27, NY CO 10, CWC27, Antigen NY-CO-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, human colon tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16502-1-AP targets SDCCAG10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SDCCAG10 fusion protein Ag9671 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serologically defined colon cancer antigen 10
别名 Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog, PPIase CWC27, NY CO 10, CWC27, Antigen NY-CO-10
计算分子量472aa,54 kDa; 291aa,33 kDa
观测分子量 54 kDa
GenBank蛋白编号BC012117
基因名称 SDCCAG10
Gene ID (NCBI) 10283
RRIDAB_1851625
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UX04
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SDCCAG10 antibody 16502-1-APDownload protocol
IF protocol for SDCCAG10 antibody 16502-1-APDownload protocol
IP protocol for SDCCAG10 antibody 16502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols