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验证数据展示

  • WB analysis of HeLa using 16073-1-AP

    WB analysis of HeLa using 16073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16073-1-AP

    IF Staining of HeLa using 16073-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16073-1-AP (SEC24C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SEC24C Polyclonal antibody

SEC24C Polyclonal Antibody for WB, IF, ELISA
Cat No. 16073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

KIAA0079, SEC24 related protein C, SEC24C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SEC24C Ready-To-Use IHC Kit. SEC24C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16073-1-AP targets SEC24C in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEC24C fusion protein Ag9030 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC24 family, member C (S. cerevisiae)
别名 KIAA0079, SEC24 related protein C, SEC24C
计算分子量 1094 aa, 118 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC018928
基因名称 SEC24C
Gene ID (NCBI) 9632
RRIDAB_2186121
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24C antibody 16073-1-APDownload protocol
IF protocol for SEC24C antibody 16073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols