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验证数据展示

  • WB analysis of Jurkat using 67409-1-Ig

    WB analysis of Jurkat using 67409-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67409-1-Ig

    IF Staining of HepG2 using 67409-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67409-1-Ig

Print datasheet

CloneNo. 3G5G9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67409-1-Ig targets SEC24D in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SEC24D fusion protein Ag4657 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SEC24 family, member D (S. cerevisiae)
Synonyms SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2882650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24D antibody 67409-1-IgDownload protocol
IF protocol for SEC24D antibody 67409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols