• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 67409-1-Ig

    WB analysis of Jurkat using 67409-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67409-1-Ig

    IF Staining of HepG2 using 67409-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67409-1-Ig

产品说明书

CloneNo. 3G5G9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67409-1-Ig targets SEC24D in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SEC24D fusion protein Ag4657 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC24 family, member D (S. cerevisiae)
别名 SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9
计算分子量 1033 aa, 113 kDa
观测分子量 113 kDa
GenBank蛋白编号BC035761
基因名称 SEC24D
Gene ID (NCBI) 9871
RRIDAB_2882650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94855
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24D antibody 67409-1-IgDownload protocol
IF protocol for SEC24D antibody 67409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols