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验证数据展示

  • IF Staining of HepG2 using CL594-67409

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:100. DAPI (blue).

  • IF Staining of HepG2 using CL594-67409

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:200.

CoraLite®594-conjugated SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for IF/ICC
Cat No. CL594-67409

Print datasheet

CloneNo. 3G5G9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67409-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67409 targets SEC24D in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen SEC24D fusion protein Ag4657 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SEC24 family, member D (S. cerevisiae)
Synonyms SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2920127
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94855
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
IF protocol for CL594 SEC24D antibody CL594-67409Download protocol
Standard Protocols
Click here to view our Standard Protocols