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验证数据展示

  • IF Staining of HepG2 using CL594-67409

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:100. DAPI (blue).

  • IF Staining of HepG2 using CL594-67409

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:200.

CoraLite®594-conjugated SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for IF/ICC
Cat No. CL594-67409

产品说明书

CloneNo. 3G5G9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC

别名

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67409-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67409 targets SEC24D in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 SEC24D fusion protein Ag4657 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC24 family, member D (S. cerevisiae)
别名 SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9
计算分子量 1033 aa, 113 kDa
观测分子量 113 kDa
GenBank蛋白编号BC035761
基因名称 SEC24D
Gene ID (NCBI) 9871
RRIDAB_2920127
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94855
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
IF protocol for CL594 SEC24D antibody CL594-67409Download protocol
Standard Protocols
Click here to view our Standard Protocols