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验证数据展示

  • IF Staining of HeLa using CL488-17913

    IF Staining of HeLa using CL488-17913

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SEC31A antibody (CL488-17913) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SEC31A Polyclonal antibody

SEC31A Polyclonal Antibody for IF/ICC
Cat No. CL488-17913

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

SEC31L1, SEC31 related protein A, SEC31 like protein 1, KIAA0905, ABP130

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SEC31A Ready-To-Use IHC Kit. SEC31A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17913-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17913 targets SEC31A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen SEC31A fusion protein Ag12314 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC31 homolog A (S. cerevisiae)
Synonyms SEC31L1, SEC31 related protein A, SEC31 like protein 1, KIAA0905, ABP130
Calculated Molecular Weight 1220 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC084583
Gene Symbol SEC31A
Gene ID (NCBI) 22872
RRIDAB_3084011
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94979
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SEC31A antibody CL488-17913Download protocol
Standard Protocols
Click here to view our Standard Protocols