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  • Featured Product
  • KD/KO Validated

SEC5/EXOC2 Monoclonal antibody, PBS Only

SEC5/EXOC2 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1C11G2

Cat No : 66011-1-PBS

Print datasheet

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p

验证数据展示

  • WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

    WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

    WB result of SEC5/EXOC2 antibody (66011-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC5/EXOC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

    IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunostaining of exocyst protein Sec5 (66011-1-Ig, red) in MDA-MB-231 cells. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    IP result of anti-SEC5/EXOC2 (IP:66011-1-Ig, 5ug; Detection:66011-1-Ig 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

    IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC5/EXOC2 antibody (66011-1-Ig, Clone: 1C11G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

产品信息

66011-1-PBS targets SEC5/EXOC2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen SEC5/EXOC2 fusion protein Ag17866 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name exocyst complex component 2
Synonyms EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p
Calculated Molecular Weight 924 aa, 104 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC016918
Gene Symbol EXOC2
Gene ID (NCBI) 55770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96KP1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.