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SEC5/EXOC2 Monoclonal antibody
SEC5/EXOC2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1C11G2
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HEK-293 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, MDA-MB-231 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2500-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66011-1-Ig targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse |
Immunogen | SEC5/EXOC2 fusion protein Ag17866 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | exocyst complex component 2 |
Synonyms | EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p |
Calculated Molecular Weight | 924 aa, 104 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC016918 |
Gene Symbol | SEC5 |
Gene ID (NCBI) | 55770 |
RRID | AB_11042317 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96KP1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SEC5/EXOC2 antibody 66011-1-Ig | Download protocol |
IHC protocol for SEC5/EXOC2 antibody 66011-1-Ig | Download protocol |
IF protocol for SEC5/EXOC2 antibody 66011-1-Ig | Download protocol |
IP protocol for SEC5/EXOC2 antibody 66011-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Nanotechnol Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.
| ||
PLoS Pathog Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape. | ||
J Cell Sci Dexamethasone-induced cellular tension requires SGK1-stimulated Sec5/GEF-H1 interaction. | ||
Int J Cancer Myoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer. | ||
Commun Biol The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium |