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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SEC5/EXOC2 Monoclonal antibody

SEC5/EXOC2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C11G2

Cat no : CL594-66011

Print datasheet

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p

验证数据展示

  • IF Staining of HeLa using CL594-66011

    IF Staining of HeLa using CL594-66011

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66011 (SEC5/EXOC2 antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66011-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66011 is SEC5/EXOC2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen SEC5/EXOC2 fusion protein Ag17866 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name exocyst complex component 2
Synonyms EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p
Calculated Molecular Weight 924 aa, 104 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC016918
Gene Symbol SEC5
Gene ID (NCBI) 55770
RRIDAB_2883476
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96KP1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

实验方案

Product Specific Protocols
IF protocol for CL594 SEC5/EXOC2 antibody CL594-66011Download protocol
Standard Protocols
Click here to view our Standard Protocols