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验证数据展示

  • IF Staining of HeLa using CL594-66011

    IF Staining of HeLa using CL594-66011

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66011 (SEC5/EXOC2 antibody) at dilution of 1:100.

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  • KD/KO验证

CoraLite®594-conjugated SEC5/EXOC2 Monoclonal antibody

SEC5/EXOC2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66011

产品说明书

CloneNo. 1C11G2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, pig, rat

应用

IF/ICC

别名

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66011-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66011 targets SEC5/EXOC2 in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, pig, rat
免疫原 SEC5/EXOC2 fusion protein Ag17866 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 exocyst complex component 2
别名 EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p
计算分子量 924 aa, 104 kDa
观测分子量 100 kDa
GenBank蛋白编号BC016918
基因名称 SEC5
Gene ID (NCBI) 55770
RRIDAB_2883476
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96KP1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

实验方案

Product Specific Protocols
IF protocol for CL594 SEC5/EXOC2 antibody CL594-66011Download protocol
Standard Protocols
Click here to view our Standard Protocols