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验证数据展示

  • WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

SECTM1 Monoclonal antibody, PBS Only

SECTM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60281-1-PBS

产品说明书

CloneNo. 1C11B4

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

K12, Protein K 12, secreted and transmembrane 1, SECTM1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

60281-1-PBS targets SECTM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SECTM1 fusion protein Ag14645 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 secreted and transmembrane 1
别名 K12, Protein K 12, secreted and transmembrane 1, SECTM1
计算分子量 248 aa, 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC017716
基因名称 SECTM1
Gene ID (NCBI) 6398
RRIDAB_2881399
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ8WVN6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.