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SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11B4

Cat no : 60281-1-Ig

Print datasheet

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

验证数据展示

  • WB analysis using 60281-1-Ig

    WB analysis using 60281-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 60281-1-Ig

    IHC staining of human breast cancer using 60281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHaCaT cells, JAR cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60281-1-Ig targets SECTM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SECTM1 fusion protein Ag14645 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name secreted and transmembrane 1
Synonyms K12, Protein K 12, secreted and transmembrane 1, SECTM1
Calculated Molecular Weight 248 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC017716
Gene Symbol SECTM1
Gene ID (NCBI) 6398
RRIDAB_2881399
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ8WVN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

实验方案

Product Specific Protocols
WB protocol for SECTM1 antibody 60281-1-IgDownload protocol
IHC protocol for SECTM1 antibody 60281-1-IgDownload protocol
FC protocol for SECTM1 antibody 60281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

iScience

SECTM1 is upregulated in immuno-hot tumors and predicts immunotherapeutic efficacy in multiple cancers

Authors - Jie Mei
View Article