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CoraLite® Plus 750-conjugated SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1C11B4

Cat no : CL750-60281

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL750-60281

    WB analysis using CL750-60281

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-60281 (SECTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

For other applications, we recommend the unconjugated version of this antibody, 60281-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-60281 targets SECTM1 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen SECTM1 fusion protein Ag14645 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name secreted and transmembrane 1
Synonyms
Calculated Molecular Weight 248 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC017716
Gene Symbol SECTM1
Gene ID (NCBI) 6398
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ8WVN6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 SECTM1 antibody CL750-60281Download protocol
Standard Protocols
Click here to view our Standard Protocols