验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
For other applications, we recommend the unconjugated version of this antibody, 60281-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-60281 targets SECTM1 in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 | SECTM1 fusion protein Ag14645 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | secreted and transmembrane 1 |
| 别名 | |
| 计算分子量 | 248 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC017716 |
| 基因名称 | SECTM1 |
| Gene ID (NCBI) | 6398 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | Q8WVN6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 SECTM1 antibody CL750-60281 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |